American Society of Hematology

Figure 2

$Figure 2. p38 isoform activity and the relative contribution of p38α and p38β in TCR-stimulated T cells. (A) T cells purified from lymph nodes of WT or p38βY323F mice were stimulated with plate-bound anti-α-CD3/α-CD28 antibodies for 30 minutes or with PMA for 10 minutes. p38β was specifically immunoprecipitated from whole cell lysates, and in vitro kinase (IVK) assays were performed with ATF-2 as substrate. (B) Unstimulated (gray filled histograms) or α-CD3/α-CD28-stimulated (solid line) T cells from mice of the indicated genotypes were stained for intracellular phospho-p38 at 30 minutes. (C) Summary of the fraction of T cells expressing phospho-p38 after stimulation with α-CD3/α-CD28, relative to WT (n = 5 mice per group).$

p38 isoform activity and the relative contribution of p38α and p38β in TCR-stimulated T cells. (A) T cells purified from lymph nodes of WT or p38β^Y323F mice were stimulated with plate-bound anti-α-CD3/α-CD28 antibodies for 30 minutes or with PMA for 10 minutes. p38β was specifically immunoprecipitated from whole cell lysates, and in vitro kinase (IVK) assays were performed with ATF-2 as substrate. (B) Unstimulated (gray filled histograms) or α-CD3/α-CD28-stimulated (solid line) T cells from mice of the indicated genotypes were stained for intracellular phospho-p38 at 30 minutes. (C) Summary of the fraction of T cells expressing phospho-p38 after stimulation with α-CD3/α-CD28, relative to WT (n = 5 mice per group).

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