Surface phenotype of IL-17⁺ Vγ9Vδ2 T cells. Vγ9Vδ2 T cells were primed for 6 days with an equal number of irradiated DCs and IPP in the presence of cytokines and then incubated for 6-7 days more in IL-2 and stimulated for 6 hours with IPP. After intracellular staining for IL-17 and IFN-γ, cells were surface stained for several different markers. (A) Chemokine receptor expression is shown on gating on IL-17⁺ or IFN-γ⁺ Vγ9Vδ2 T cells. The vertical line in each panel indicates the negative cutoff as determined by staining with isotype-control mAbs. (B) Surface markers expression on IL-17⁺ Vγ9Vδ2 T cells (violet-filled lines). Green open lines indicate staining with isotype-control mAbs.