Plasma and liver AAT levels. (A) AAT antigen is increased in Lman1^−/− mouse liver lysates. Liver lysates were prepared from WT and Lman1^−/− mice and analyzed for AAT, TRAPα, and β-actin by immunoblotting. Relative levels of the lower band of AAT were quantified and normalized to β-actin. (B) AAT accumulates in the ER of Lman1^−/− mouse liver. Liver lysates from WT and Lman1^−/− mice were digested with endo H and analyzed for AAT, albumin, CatZ, and β-actin by immunoblotting. Each lane contains a sample from a different individual mouse.