Broad recognition of tumor cells, but not of the normal tissues, is dependent on mevalonate-pathway intermediates. (A) Tumor cell lines and normal tissue-derived cells were loaded with ⁵¹Cr and incubated with γ9δ2TCR-transduced αβT cells at E:T ratio 10:1 for 4-5 hours. Percentage of the specific lysis is shown as the mean of at least 3 independent replicates for each target. (B) Various target cells, as indicated, were subjected to the lysis by γ9δ2TCR-transduced αβT cells at E:T ratio of 10:1 after treatment with 25μM mevastatin, 10μM pamidronate, with 15μM IPP or untreated. Shown is a summary of fold change of the specific lysis of treated (□, mevastatin;, pamidronate; and ■, IPP treatment) versus untreated target in at least 3 independent experiments for each cell line. WT1-specific TCR-transduced αβT cells served as a control for mevastatin/pamidronate/IPP-independent specific lysis of T2 cells loaded with wt1 peptide. Significance was determined by 2-way ANOVA analysis; *P < .01; **P < .001.