Quantification of miR-223* and miR-223* targets. (A) Venn diagram comparing differentially expressed genes (shown as transcript cluster IDs), assessed by mRNA microarrays with predicted mRNA targets (shown as transcript cluster IDs). (B) Quantification of Cux1, Igf1r, Dlc1, and Pik3cd by real-time PCR in miR-223KO cells expressing strand-inactivating constructs (n = 3). ctl indicates control. (C) IGF1R luciferase assay. neg CTL indicates negative control. (D) Comparison of miR-223 and miR-223* levels in healthy donors (n = 10) and AML patients with normal cytogenetics (n = 92). The y-axis depicts ΔCT (miRNA−miR-92) values. (E) miR-223* levels measured in 92 AML patients with normal cytogenetics by real-time PCR. Overall survival was plotted on miR-223* levels dichotomized to the 75th percentile. *P < .05