Deep-sequencing profile of 10 miRNA libraries. (A) Examples of miRNA/miRNA* duplexes with high, intermediate, and low ratios across all tissues. The x-axis indicates all sequencing libraries, and the y-axis indicates all calculated miRNA:miRNA* ratios. (B) Examples of miRNA duplexes with dynamic arm expression. The x-axis indicates all sequencing libraries, and the y-axis indicates all calculated miRNA:miRNA* ratios. (C) Experimental setup to test the activity of miR-223 and miR-223* in colony-forming cell assays. (D) Colony counts for each experimental arm (n = 3). ES undiff indicates undifferentiated embryonic stem cells; ES diff, differentiated embryonic stem cells; LTR, long terminal repeat; pgk, phosphoglycerate kinase; GFP, green fluorescent protein; CFC, colony-forming cell; and MIG, MSCV (murine stem-cell virus)–IRES-GFP.