Reconstitution of DCs, numbers, and phenotype in the [B6 → B6Ly5.2] and [Ik^−/− → B6 Ly5.2] chimeras. WT B6 Ly5.2 animals were lethally irradiated with 11 Gy and infused with 5 × 10⁶ BM cells and 5 × 10⁶ splenocytes from syngeneic Ly5.1 WT B6 or Ik^−/− B6 donors. Four months later, these animals (n = 4-5 animals/group) were killed and the splenocytes were harvested and analyzed for total splenocyte count (A), frequency and number of CD11c⁺ DCs (B), and frequency and absolute numbers of CD8⁺CD11c⁺ DCs and CD8⁻CD11c⁺ DCs (C). The CD8⁺CD11c⁺ and CD8⁻CD11c⁺ DCs were analyzed for expression of CD80 (D), CD86 (E), CD40 (F), CD83 (G), and B7H1 (PD-L1) (I) I-Ab. *P < .05, **P < .01.