Splenic DC phenotype in the absence of Ikaros. The total splenocyte count and the frequency and the expression of costimulation molecules on CD11c⁺ cells were analyzed from naive Ik^−/− and WT B6 animals that were not transplanted (n = 5-7/group). *P < .05; **P < .01; ***P < .001. (A) Total splenocyte count. (B) Expression of CD11c and CD8. The black and gray boxes signify CD8⁺CD11c⁺ DCs and CD8⁻CD11c⁺ DCs, respectively. (C) Frequency and absolute number of CD11c⁺ DCs. (D) Frequency and absolute number of CD8⁺CD11c⁺ DCs and CD8⁻CD11c⁺ DCs in spleen. The expression of the costimulatory molecules CD80 (E), CD86 (F), CD40 (G), CD83 (H), and B7H1 (PDL1) I-A^b (I) on CD8⁺CD11c⁺ DCs or CD8⁻CD11c⁺ DCs.