Reappearance of BRIC6 (band 3) and LA1818 (RhAG) epitopes after pronase treatment occurs most rapidly at the early stages of erythropoiesis. (A-B) Assessment of recovery of BRIC6 (band 3; A) and LA1818 (RhAG; B) epitopes on cells pretreated with 500 μg/mL pronase for 0, 60, 120, and 180 minutes, as measured by flow cytometry (mean fluorescent intensity [a.u., arbitrary units] in differentiation medium at 37°C at indicated periods of time during differentiation [hours]). Means and SDs represent 3 experiments. (C) Cells were pretreated with 100 ng/mL CHX (□, ▵, ♢, ○) or not (■, ▴, ♦, ●) before pronase treatment. Recovery of BRIC6 (band 3) and LA1818 (RhAG) epitopes was monitored at 0, 60, 120, and 180 minutes after pronase treatment at specific time points during differentiation. Data are expressed as mean fluorescence intensity (a.u.) as a function of recovery time from 2 independent experiments.