pDCs from HIV infected DKO-hu mice are functionally impaired. (A) Relative HIV-1 replication level in the blood at 1 and 2 weeks after infection was shown. (B) IFNα in the plasma of mock or HIV-infected DKO-hu mice was measured by the Human Cytokine Luminex kit. Error bars are SD (n = 4). *P < .05. (C-D) BM cells from mock or HIV-infected DKO-hu mice were stimulated with HSV for 16 hours. The expression of intracellular IFNα in pDCs were measured by FACS (C). (D) Summarized data from 4 mock and 4 HIV-infected DKO-hu mice are shown. (E-F) BM cells were also stimulated with the influenza virus and analyzed for the expression of IFNα in pDCs. (F) Summarized data from 4 mock and 4 HIV infected DKO-hu mice are shown. Error bars represent SD. *P < .05.