Development of pDC in lymphoid organs in DKO-hu mice. (A) Total live human cells (VLD-mCD45⁻hCD45⁺) from BM or spleen (SP) were analyzed for CD123 expression relative to BDCA2, CD4, HLA-DR, and CD3 expression. (B) pDCs in different lymphoid organs from the DKO-hu mice were analyzed. The number in each plot represents the percentage of CD4⁺CD123⁺ pDCs of total human CD45⁺ cells. Human PBMCs were used as a control. (C) Summarized data show average percentage of pDCs (CD3⁻CD4⁺CD123⁺ of total human CD45⁺ cells) in different lymphoid organs from DKO-hu mice at 12 weeks after transplantation (n = 7). (D) Total numbers of pDCs in each lymphoid organ are calculated as described in “Flow cytometry.” Error bars indicate SD (n = 7).