Impact of KC depletion on innate immune response 2 hours after liver gene transfer with scAAV2 vector in C3H/OuJ mice (n = 4 per experimental group). (A) Fold-changes in hepatic mRNA levels (compared with PBS-injected mice) are shown for mice that did or did not receive GdCl₃ treatment before scAAV2-GFP vector administration. A change > 2.5-fold (horizontal line) was considered greater than the variability of the assay. (B) Cytokine levels of TNF-α, IL-6, and MCP-1 in liver protein extract. (C) Systemic IL-6 levels. *P < .05 in comparison with ssAAV2-transduced liver as calculated by 2-tailed Student t test.