Identification of Rac1-Bcl-2 interaction. Various amounts of recombinant Bcl-2 (A), Bcl-xL (B), and Bax (C) were incubated with GST-Rac1. The membranes were probed with the respective antibodies (anti-Bcl-2, anti–Bcl-xL, or anti-Bax). Recombinant proteins alone were used as positive controls. GST-Rac1 alone was used as a negative control. The data are representative of 4 independent experiments.