DC_Th subsets promote distinct T_H cell responses. CD14⁺ monocytes were cultured with IL-2 and allogeneic T_H1 cells (DC_Th1), T_H2 cells (DC_Th2), or T_H17 cells (DC_Th17). (A) On day 6 of culture, each DC_Th coculture was stimulated with LPS for 36 hours. Supernatants were subsequently collected for measurement of IL-1β, IL-6, IL-10, IL-12p70, IL-23p19, and TNF-α by ELISA. Data are from 5 independent experiments and > 10 donors (mean and SEM), *P < .05; **P < .01; ***P < .001. (B) T cell–depleted DC_Th subsets from autologous cocultures were stimulated with LPS at day 6 for 36 hours and subsequently cultured with allogeneic naive CD4⁺ T cells in an MLR. After 6 days, supernatants were collected for measurement of IL-5, IL-13, IL-17A, and IFN-γ by ELISA. Data are from 4 independent experiments and 4 donors (mean and SEM), *P < .05, **P < .01 compared with all other subsets. See also supplemental Figure 4.