TH subsets instruct monocytes to form specialized DC subsets. (A-B) Representative skin lesions from psoriasis and atopic dermatitis patients were stained with Abs against T-bet (left column), RORγ (middle column) or GATA-3 (right column) and (A) CD4 or (B) CD14. Images are shown at 400× magnification. Red arrows indicate examples of (A) double positive cells or (B) monocyte/TH cell interactions. Scale bar equals 20 μm. (C) CD14+ monocytes were cultured with IL-2 and allogeneic TH1 cells (DCTh1), TH2 cells (DCTh2) or TH17 cells (DCTh17). After 6 days, DCTh subsets were analyzed for surface expression of DC-associated molecules. MFIs are shown after excluding TH cells. Dashed lines indicate the average MFIs of isotype control Abs used for staining from all culture conditions. Data are from 5 independent experiments and > 10 donors (mean and SEM), *P < .05; **P < .01; ***P < .001.