Figure 4
IGFBP2 supports the survival and cycling of HSCs in the BM. (A) Lin−Sca-1+Kit+ cells from BM of WT or IGFBP2-null mice were analyzed for early apoptosis (annexinV+7-AAD−) and late apoptosis (annexinV+7-AAD+) markers (n = 6). (B) Cell cycle status of Lin−Sca-1+Kit+Flk2+CD34+ cells and (C) Lin−Sca-1+Kit+Flk2−CD34− cells was evaluated by Hoechst 33 342 and pyronin Y staining (n = 6). (D) BrdU incorporation into Lin−Sca-1+Kit+ cells was measured (n = 8). (E) The expression of certain growth and survival related genes in freshly isolated BM Lin−Sca-1+Kit+ cells was evaluated by real-time RT-PCR (n = 4-6).

IGFBP2 supports the survival and cycling of HSCs in the BM. (A) LinSca-1+Kit+ cells from BM of WT or IGFBP2-null mice were analyzed for early apoptosis (annexinV+7-AAD) and late apoptosis (annexinV+7-AAD+) markers (n = 6). (B) Cell cycle status of LinSca-1+Kit+Flk2+CD34+ cells and (C) LinSca-1+Kit+Flk2CD34 cells was evaluated by Hoechst 33 342 and pyronin Y staining (n = 6). (D) BrdU incorporation into LinSca-1+Kit+ cells was measured (n = 8). (E) The expression of certain growth and survival related genes in freshly isolated BM LinSca-1+Kit+ cells was evaluated by real-time RT-PCR (n = 4-6).

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