Treatment with PTEN inhibitor SF1670 increases chemoattractant-elicited superoxide production in both human and mouse neutrophils. (A-F) Human neutrophils were treated with SF1670 at 37°C for 30 minutes and then washed twice with HBSS. Production of ROS was monitored after stimulation with indicated concentrations of fMLP in the presence of 50μM isoluminol, 0.8 U of horseradish peroxidase, and 0.2% bovine serum albumin in a luminometer at 37°C. Chemiluminescence (arbitrary light units) was recorded at indicated times. Data are mean ± SD from 1 experiment representative of 3 experiments. (G-J) Bone marrow–derived mouse neutrophils were treated 500nM SF1670 at 37°C for 30 minutes, washed twice with HBSS, and then stimulated with indicated concentrations of fMLP. ROS production was monitored in the presence of 50μM isoluminol, 0.8 U of horseradish peroxidase, and 0.2% BSA in a luminometer as described here. Data are mean ± SD from 1 experiment representative of 3 experiments.