YW152F treatment affects tumor vessel morphology and the number of GFP⁺, α-SMA⁺, desmin⁺, and NG2⁺ cells. TC71 cells were subcutaneously injected in nude mice that had previously received GFP⁺ BM transplants. Mice were treated twice weekly with YW152F or IgG for a total of 5 treatments. Mice were then killed and tumors were analyzed by immunohistochemistry. (A) CD31⁺ endothelial cell structures. The number of open lumens per 10× field was manually counted. The average of 5 fields was determined for each tumor. (B) Anti-GFP was used to identify BM-derived cells; α-SMA, desmin, and NG2 were used to identify pericytes/vSMCs. Five 10× fields from each tumor were used to quantify cell numbers using Simple PCI software. The percentage reduction in the average positive pixel/nuclei ratio in YW152F-treated tumors compared with IgG-treated tumors was determined.