Effect of o-vanadate on the microscopic and macroscopic diffusion coefficients of band 3 in intact erythrocytes. Distributions of the logarithms of D_micro and D_Macro obtained from single particle tracking measurements on intact control cells (top row) and o-vanadate–treated cells (1mM, middle row; 2mM, bottom row). As anticipated, the cells used for single-particle diffusion analyses displayed the same morphologic changes as a function of time as the similarly treated cells shown in Figure 4A. Gaussian fits were performed on the histograms to determine the mean values of the distributions and their abundances. The number of Gaussian distributions required to fit the histograms was chosen using the F test with a 95% confidence level.