Donor Langerhans cells engraft in Langerin-DTA mice independent of donor T cells. (A-B) Flow cytometric analysis of LCs. LCs are present in ears of Langerin-DTA mice transplanted with T cell–depleted C3H.SW BM (A) (representative flow cytometry). Donor LCs engraft in Langerin-DTA recipients of Tg⁻ BM (B third panel) or RAG1^−/− BM (B fourth panel). The first 2 panels show data from unmanipulated B6 and Tg⁺ control mice. Data are representative of 3-6 mice per group from at least 2 independent experiments with similar results. (C) Epidermal preparations from B6 controls or RAG1^−/−→Tg⁺ transplantation recipients were stained for Langerin (red, first column) and MHCII (green, second column); the overlay of Langerin and MHCII expression is shown in the third column. Note the patchy engraftment of donor LCs in RAG1^−/−→Tg⁺ recipients.