TLR2 ligand increases inflammatory-related gene and protein expressions. (A-D) Meg-01 cells were pretreated for 45 minutes with 50μM LY294002, U0126, BAY11-7082, or DMSO, and then treated for 3 hours with 1 μg/mL of Pam3CSK4 (PAM). MCP-1 (A), COX2 (B), NFκB1 (C), and TLR2 (D) gene expression was compared with NT, which was set at 100%. Insets in panels B and C show Meg-01 cells treated with 1 μg/mL of Pam3CSK4 for 3 hours. Western blots were probed for COX2 (B), NFκB105/50 (C), and actin (loading controls). Western blots are representative of 3 independent experiments. *P < .05, **P < .01, and ***P < .001 compared with NT (n = 12 in panel A; n = 9 in panel B; n = 8 in panel C; and n = 10 in panel D).