Epigenetic regulation of miR-29a. (A) MiR-29a expression levels by RT-qPCR with or without 5-aza-2′-deoxycytidine (5-aza-DC) treatment in Karpas-299, SU-DHL-1 (ALK⁺) and FE-PD (ALK⁻) cells are shown. Data represent the mean of triplicates with standard deviations (SD) and *P < .05. (B) Schematic representation of miR-29 locus on chromosome 7, coding for miR-29b1 and miR-29a (black boxes). The prediction of a CpG-enriched site with Emboss CpGPlot software (http://www.ebi.ac.uk/Tools/emboss/cpgplot/index.html) is represented as a dashed box and contains 5 CpG sites. Bisulphite sequencing of these 5 CpG sites in cells treated with 5-aza-DC or transfected with siALK, and control ALCL cells. Each circle indicates a CpG dinucleotide (●: methylated CpG; ○: unmethylated CpG).