MiR-29a is low expressed in ALK⁺ ALCL. (A) MiR-29a expression in ALK⁺ (SU-DHL-1, COST, PIO, Karpas-299) and ALK⁺ ALCL cells (FE-PD) was analyzed by RT-qPCR and Northern blotting. RNA from reactive lymph node (RLN) and peripheral blood mononuclear cells (PBMC) serve as normal controls. The histogram shows the relative expression (RQ) of miR-29a after normalization with RNU24. Bars represent standard deviations (SD). P for ALK⁺ vs controls and ALK⁺ vs ALK⁻ (*P < .05). 5.8S RNA was used as the loading control in the Northern blotting. P for ALK⁺ vs controls (P < .001) and ALK⁺ vs ALK⁻ (P < .05). (B) RT-qPCR of miR-29a in 20 ALK⁺ and 12 ALK⁻ patient tumors. Three RLNs were used as normal controls. RQ was plotted on a logarithmic scale. P for ALK⁺ vs RLN and ALK⁺ vs ALK⁻ (***P < .001).