Bortezomib inhibits the nuclear translocation of IRF-7 and NF-κB in pDCs. Purified pDCs were cultured in the absence or presence of 10nM bortezomib for 3 hours and stimulated with 0.5μM ODN2216 for 3 hours. The cells were stained with rabbit anti–IRF-7 (A) or NF-κB p65 (B) and with Alexa Fluor 488–conjugated goat anti–rabbit immunoglobulin G as a secondary antibody. Nuclei were identified using TOTO-3 dye. The data are representative of 3 experiments.