Bortezomib does not inhibit the trafficking of Unc93B1 from the ER to endolysosomes induced by CpG ODN. M12 expressing Unc93B1-GFP was cultured in the absence or presence of 30nM bortezomib for 1 hour and cultured with 0.5μM ODN1668 in the absence or presence of bortezomib for 2 hours. ER-Tracker (A) and LysoTracker (B) were added during the last 30 minutes. The cells were observed by confocal microscopy. The data are representative of 4 experiments.