Bortezomib inhibits the trafficking of TLR9 from the ER to endolysosomes induced by CpG ODN. The mouse B-cell line M12 expressing TLR9-GFP was cultured in the absence or presence of 30nM bortezomib for 1 hour and cultured with ODN1668 in the absence or presence of bortezomib for 2 hours. ER-Tracker (A) and LysoTracker (B) were added during the last 30 minutes. The cells were observed by confocal microscopy. The data are representative of 4 experiments.