Iron homeostasis in Hjv skeletal muscle–specific knockout mice on iron-rich diet. (A) Serum iron concentration. (B) Serum transferrin saturation. (C) Nonheme tissue iron concentrations (μg/g wet weight) of Hjv fl/fl and skeletal muscle–specific knockout (sk/sk) male mice. L indicates liver; and S, spleen. (D) Nonheme iron concentrations in skeletal muscle. (E) Hepatic Hamp1 relative to Rpl19 mRNA expression. (F) Hepatic Id1 relative to Rpl19 mRNA expression. Mean values from analysis of 8-week-old male mice (n = 4 for fl/fl and n = 6 for sk/sk) are graphed. Error bars represent SD. No statistically significant differences were observed.