Iron homeostasis in Hjv skeletal muscle–specific knockout mice on iron-deficient diet. (A) Serum iron concentration. (B) Serum transferrin saturation. (C) Nonheme tissue iron concentrations (μg/g wet weight) of Hjv fl/fl and skeletal muscle-specific knockout (sk/sk) female mice. L indicates liver; S, spleen; and SK, skeletal muscle. (D) Hepatic Hamp1 relative to Rpl19 mRNA expression. Note that all values were very low compared with mice on a standard diet. (E) Hepatic Id1 relative to Rpl19 mRNA expression. Mean values from analysis of 8-week-old female mice (n = 8 per genotype) are graphed. Error bars represent SD. No statistically significant differences were observed.