Hjv liver-specific knockout mice exhibit iron-overload phenotypes. (A) Relative Hjv mRNA expression in liver determined by quantitative RT-PCR. fl/fl indicates mice with the homozygous fl allele; l/l, homozygous fl mice expressing the Cre transgene in liver. The Hjv expression level in the livers of fl/fl mice was assigned a value of 1. (B) Serum iron concentration. (C) Serum transferrin saturation. (D) Nonheme tissue iron concentrations (μg/g wet weight) of fl/fl and Hjv liver-specific knockout (l/l) mice. L indicates liver; S, spleen; H, heart; and SK, skeletal muscle. (E) Hepatic Hamp1, Id1, and Bmp6 relative to Rpl19 mRNA expression by quantitative RT-PCR. Results are reported as the -fold change from Hjv fl/fl mice. (A-E) Mean values from analysis of 8-week-old mice (fl/fl mice: 3 male and 2 female, l/l mice: 4 male and 2 female) are graphed. Error bars represent SD. ‡P < .05, #P < .005, and §P < .0005 compared with Hjv fl/fl mice.