β-glucan phagocytosis and binding on DCs and macrophages. BMDCs (A) and macrophages (B) were incubated with DTAF-WGP at 37°C and phagocytosis of particulate β-glucan was assessed by flow cytometry and ImageStream. Percent of particulate β-glucan positive cells were summarized. One representative dot plot from 3 independent experiments with similar results. (C) BMDCs and macrophages were incubated with PGG-DTAF β-glucan on ice and β-glucan binding was assessed by flow cytometry. The data indicate that soluble β-glucan binding on DCs and macrophages is independent of dectin-1 or CD11b receptors. (D) BMDCs and macrophages were incubated with soluble PGG β-glucan or dextran and then mixed with DTAF-WGP on ice. Particulate β-glucan binding on DCs or macrophages was measured by flow cytometry. Cells were gated on CD11c⁺ or F4/80⁺ cells.