Figure 3.
![Figure 3. Evidence of eNOS expression in RBCs. (A) Western blot analysis revealed an eNOS-specific band at 140 kDa in preparations of RBCs (lanes 2 and 3 are derived from diluted preparations [Hct 20%, 10%, and 5%, respectively; with 15, 7.5, and 3.8 μg protein/lane]). This band is equivalent to positive controls of human aortic endothelial cells (HAECs; 2 μg protein/line) and myocardium (cell lysate; 1 μg protein/line). (B) The 450-bp fragment of eNOS mRNA from RBCs corresponds to the control of HUVECs and whole blood. The purity of the RBC fraction was confirmed by mRNA amplification of reference proteins: endoglin for HUVECs, β-globin for RBCs, β-3 integrin for platelets, and CD45 for WBCs. Whole blood was taken as positive control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/7/10.1182_blood-2005-10-3992/5/zh80070693530003.jpeg?Expires=1723736741&Signature=AiU8jPxMjXynmnxOA3txUvep9I9j0GfNb8uqJ~aXJugGcs-M6pDe-xNclgTzlvjxlzbJ-wmf-JtHL5CUvuLqFA5O2ncmhzQNYkqOvnzlf341xDXLInXfsZr3j1eqzTWOHALEwzivbfPr2ur9oIq1UF-bZ~8HsZYMQ5VVDUg700PZIxcNd315H5CZk1CBNhb13w9HzsJoW3pBzk9qAnw~yJfyU9z3N6JmXPssDmfwvP3TJQOspL7Lz5qgqTUsBxa~gfY14-aBQzfH1w1F~k-jBzn2xY89ZjvwpUhy3XVNQOQ2igAf1FJvMvmfF1G2bjuVQMcUScjf25E3wnFFnw7~BA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Evidence of eNOS expression in RBCs. (A) Western blot analysis revealed an eNOS-specific band at 140 kDa in preparations of RBCs (lanes 2 and 3 are derived from diluted preparations [Hct 20%, 10%, and 5%, respectively; with 15, 7.5, and 3.8 μg protein/lane]). This band is equivalent to positive controls of human aortic endothelial cells (HAECs; 2 μg protein/line) and myocardium (cell lysate; 1 μg protein/line). (B) The 450-bp fragment of eNOS mRNA from RBCs corresponds to the control of HUVECs and whole blood. The purity of the RBC fraction was confirmed by mRNA amplification of reference proteins: endoglin for HUVECs, β-globin for RBCs, β-3 integrin for platelets, and CD45 for WBCs. Whole blood was taken as positive control.
