Role of the FasL-interacting proteins Lck and Fyn in lipid raft recruitment. 293T cells were cotransfected with full-length FasL and vector control, Lck, or Fyn. Lipid rafts were isolated, and FasL, Lck, Fyn, and flotillin were detected by Western blot. NR indicates nonraft fraction; R, raft fraction. A typical experiment of 5 is shown.