The effect of PIAS3_82-132 on STAT3- and MITF-mediated gene expression. (A) Quantitative real-time PCR analysis of MITF target genes (c-Kit and Tbx2), STAT3 target genes (CyclinD1 and VEGF), and MITF and STAT3 target gene (c-fos) from transfected melanocytes. (B) Quantitative RT-PCR analysis of MITF mast cell target genes (GrB and TRP) from transfected mast cells. In both cases, cells were transfected with expression plasmids containing PIAS3_1-628, PIAS3_82-132, or an empty vector. Total RNA (1.7 μg) was used from each transfection experiment. Transcript levels of MITF and STAT3 target genes were normalized to actin and performed in triplicate.