Plinabulin treatment leads to mitotic block in MM. (A) MM cell lines MM.1S and RPMI-8226 were treated with plinabulin (8nM) for 6, 24, and 48 hours, followed by analysis for histone H3 phosphorylation with the use of flow cytometry. Data are representation of 3 independent experiments. (B) MM.1S cells were treated with plinabulin (8nM) for 24 hours and stained for α-tubulin, pericentrin, and Hoechst. (C) Fluorescence intensity (FI) of α-tubulin (green fluorescence) was measured with the use of Velocity software (Improvision). Representative figures (C-D) are from 4 independent experiments. Error bars indicate SD.