Inhibition of angiogenesis by Eap in the in vivo matrigel plug assay. (A) Neovascularization in the matrigel plug assay was performed without (buffer) or with VEGF (100 ng/mL) in the absence or presence of 10 μg or 30 μg Eap as indicated. Photographs of a typical experiment performed in triplicates are shown; similar results were obtained in 3 separate experiments. (B) The quantitation of neovascularization in the matrigels was performed by measuring the hemoglobin concentration. Hemoglobin concentration was expressed as milligrams of hemoglobin per grams of wet tissue. Data are expressed as a percentage of the maximum (VEGF treatment in the absence of competitors). Data are means ± SD (n = 3) of a typical experiment; similar results were observed in 3 separate experiments.