Inhibition of leukocyte infiltration into wounds by Eap. (A-B) Typical photomicrographs of immunohistochemical staining for the detection of neutrophils (anti-MPO) at day 1 following wound generation in the absence (A; buffer) or presence of 20 μg Eap (B) is shown. (C) The expression of MPO at day 1 following wound generation as assessed by Western blot is shown in the absence (□) or in the presence (▪) of Eap. The insert demonstrates a typical blot with staining for MPO. Densitometric data are expressed relative to control (buffer-treated wounds), and are means ± SD of 3 separate experiments. (D-E) Typical photomicrographs of immunohistochemical staining for the detection of macrophages (anti-F4/80) at day 5 following wound generation in the absence (D; buffer) or presence (E) of Eap is shown. (F) The expression of tissue factor at day 5 after wound generation as assessed by Western blot analysis is shown in the absence (□) or presence (▪) of Eap. The insert demonstrates a typical blot. Densitometric data are expressed relative to control (buffer-treated wounds), and are means ± SD of 3 separate experiments.