CD4 RNA spectratyping shows oligoclonality in subject 1 before transplantation and polyclonality in both subjects after transplantation. Subject 1 at (A) day −127 and (B) day 873 after transplantation; subject 2 at (C) day 368 after transplantation. The D_KL score for subject 1 pretransplantation (A) is 1.38 compared with the D_KL score of 0.19 after transplantation (B). For subject 2, the posttransplantation D_KL was 0.08 (C). Lower D_KL scores reflect greater diversity in the TCR repertoire. The “X” indicates panels with insufficient RNA concentrations. These panels were not included in the calculation of the D_KL score. NC indicates negative control.