Figure 1.
Hypoxia induces cord formation in HUVECs. (A) HUVECs were seeded on growth factor–reduced Matrigel in GF+ or in GF– medium. Cells were then cultured under 21% O2 (normoxia) or 1% O2 (hypoxia) for 16 hours. Cord formation was examined by phase-contrast microscopy at × 10 magnification. Results are representative of 3 independent experiments. (B) HUVECs were cultured as described in panel A. Every well was divided in 3 different parts, and the length of cords was counted in each part using the Bioquant Image Analysis System.
