Figure 3
![Effects of PGE1 on platelet activation and fibrin formation. Platelets were superfused with plasma at 250 s−1 in the absence (control) or presence (0.5μM) of PGE1. Bright-field and contrast images (Nikon Diaphot 200 microscope, see “Methods”) were taken at 0-60 seconds after start of fibrin formation (bars, 10 μm). (A) Reduced fibrin on platelets after PGE1 treatment (average pixel intensities of subtracted images). (B) Lower [Ca2+]i rises with PGE1 in Fluo-4–loaded platelets. (C) Lower OG488–annexin A5 staining with PGE1. (D) Reduced OG488-fibrin(ogen) staining (integrated fluorescent intensities). Means ± SE; n ≥ 3; *P < .05, ***P < .001 vs control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/2/10.1182_blood-2010-01-262683/4/zh89991064420003.jpeg?Expires=1720284516&Signature=q6R1xq0PL~FXq-zkQs90L44s5Iwrm~qCVkoZc1vHFWQ7SmssIOipg8dQmEtVnb3AIv5jrwg4dd0DJcPjWVw8dSmhPHAPdo0rKWKJBHdxnhn7e8T~vMGsfrsPhPWnPx1pCmbcz19sS9d8dFEmpDCZEQVcAvemkeFZfoJjI93G7lx~v~g-5BXAdCzh5u1WMHsdiW5LfWZd32A3LX7G2TNbG7gNztLbWUsfGaac61A3WIm6xHYaf0NN4REaaSToVQihzHx3fSK2oh0GfoM9IFDXNn0ZP0WpIZGzMTotB7c943kN4~Ckdtele~tigg-JQhw7w0H3zF1~zt2BIbEXO0y00w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of PGE1 on platelet activation and fibrin formation. Platelets were superfused with plasma at 250 s−1 in the absence (control) or presence (0.5μM) of PGE1. Bright-field and contrast images (Nikon Diaphot 200 microscope, see “Methods”) were taken at 0-60 seconds after start of fibrin formation (bars, 10 μm). (A) Reduced fibrin on platelets after PGE1 treatment (average pixel intensities of subtracted images). (B) Lower [Ca2+]i rises with PGE1 in Fluo-4–loaded platelets. (C) Lower OG488–annexin A5 staining with PGE1. (D) Reduced OG488-fibrin(ogen) staining (integrated fluorescent intensities). Means ± SE; n ≥ 3; *P < .05, ***P < .001 vs control.
