Pofut1^+/−/FX^−/− mice have an impaired response to exogenous fucose. (A) Peripheral blood T cells, B cells, and granulocytes were analyzed by FACS in 8- to 12-week-old Pofut1^+/+FX^+/+ (Pofut1^+/+) (n = 10) and Pofut1^+/−FX^+/+ (Pofut1^+/−) mice (n = 11). (B-F) Pofut1^+/−/FX^−/− or Pofut1^+/+/FX^−/− mice were maintained on fucose-supplemented chow (0.5% fucose weight/weight) until 8 weeks old and then maintained on standard chow without fucose supplementation for 1 month. Pofut1^+/−/FX^−/− or Pofut1^+/+/FX^−/− mice were then fed water containing various concentrations of fucose. Four weeks later, PB and marrow were analyzed by FACS. Control group mice (no fucose) (n = 4) were maintained on standard chow for 4 weeks. (B) Absolute neutrophil numbers in the periphery 4 weeks after mice were fed fucose water or maintained on standard chow. (C-E) Absolute neutrophil numbers in Pofut1^+/−/FX^−/− or Pofut1^+/+/FX^−/− mice at different times after mice were fed 1mM (C; n = 5), 2.5mM (D; n = 5), or 5mM (E) fucose water (n = 6). (F) Marrow myeloid progenitor profiles were obtained from Pofut1^+/−/FX^−/− or Pofut1^+/+/FX^−/− mice 4 weeks after fucose supplementation and compared with those in mice receiving no-fucose chow, or in mice with Pofut1^+/−/FX^+/+ or Pofut1^+/+/FX^+/+ genotype. Shown is 1 representative profile of at least 4 similar analyses. The percentages of CMP or GMP cells of Lin⁻Sca-1⁻IL-7R⁻c-kit⁺ compartment and the ratios of GMP/CMP are summarized in Table 1. Student t test was performed to compare the cell numbers from Pofut1^+/− (FX^−/−) with those from Pofut1^+/+ (FX^−/−) mice, or from Pofut1^+/−/FX^−/− mice with those from Pofut1^+/+/FX^−/− (A-E). Data are mean ± SD. P values that are significantly different are shown as indicated.