Mx-Cre/Pofut1^F/F (Mx-Cre/P^F/F) marrow LSKs have reduced T lymphopoiesis but enhanced myelopoiesis in vitro in response to Dll4 activation. (A-B) A representative FACS profile of 3 similar experiments on day 20 culture of Mx-Cre/P^F/F or control marrow LSK cells from 12-week-old mice (8 weeks after pIpC injection), cocultured with OP9-control cells (no ligand; Ret10) or OP9-Dll4 (Dll4) in the presence of dimethyl sulfoxide (DMSO) (A) or 10μM γ-secretase inhibitor (GSI) (B), for CD8 and Gr-1 expression. (C) A representative FACS profile of Mx-Cre/P^F/F and control LSKs from 12-week-old mice (8 weeks after pIpC injection) expressing Notch receptors detected by PE-conjugated antibodies against mouse Notch 1, Notch2, Notch3, or isotype control. Mean fluorescence intensity (MFI, mean ± SD) of each genotype is included in parentheses. Student t test was performed to compare the MFI from Mx-Cre/P^F/F LSKs to that from the control LSKs. * P < .05. (D) A representative FACS analysis from 3 experiments to determine recombinant Notch ligand Dll1 and Dll4 binding to Mx-Cre/P^F/F and control LSKs in the presence of 0.01 g/L of Ca⁺⁺, or in the presence of 10mM ethylenediaminetetraacetic acid.