Impaired T- and MZB-cell development in Mx-Cre/Pofut1^F/F mice. (A-B) Representative gross anatomy of thymus (A) and total thymocyte numbers per lobe from 16-week-old, pIpC-treated control or Mx-Cre/Pofut1^F/F mice (n = 6) (B). (C) FACS analysis of CD4 versus CD8 on total thymocytes, as well as histograms for TCR-αβ and TCR-γδ expression on DN4 thymocytes, from pIpC-treated control and Mx-Cre/Pofut1^F/F mice. Percentages of CD4⁺, CD8⁺, CD4⁺CD8⁺ (DP), and CD4⁻CD8⁻ (DN) are indicated in the quadrants. (D) Absolute numbers of thymocyte subsets per lobe described in panel C and intrathymic B220⁺ cells were calculated and shown as bar diagrams (n = 5). (B,D) Bar graphs represent the mean ± SD. Student t test was performed to compare the absolute number of cells from Mx-Cre/Pofut1^F/F mice with those of control mice. *P < .05. **P < .01. (E-F) FACS analysis of spleen IgM versus IgD expression on B220⁺ cells (E), and CD21 versus CD23 expression on B220⁺ cells (F) in control and Mx-Cre/Pofut1^F/F mice. (C,E-F) Data are representative of 4 independent experiments with 1 or 2 mice of each genotype per experiment.