Epo reduces the number of vessels in the BM microenvironment. (A-C) Representative sections (original magnification ×100) in the secondary spongiosa on paraffin sections of tibiae from PBS and Epo-treated mice for immunohistochemistry for the endothelial marker VEGFR3. v indicates vessel; tb, trabecular bone; and m, marrow. (D) Number of vessels per marrow area (total area − bone area). (E) Vessel area per vessel. (F) Total perimeter of vessels per marrow area. (G) Total volume of vessels per marrow area. n = 6 and 5 for PBS and Epo treatment, respectively. Data are mean ± SEM.