Figure 3
Increased number of HSCs and committed progenitor cells and EPO-independent erythroid colony formation in ΔHmga2 mice. (A) Proportions (left) and absolute numbers (right) of KLS cells and committed progenitor cells in total BM cells as determined by flow cytometry (n = 4 in each). (B) Numbers of BFU-Es and CFU-GMs grown from 1 × 104 BM cells (n = 8 in each) and 1 × 106 spleen cells (n = 4 in each) in the presence of human EPO and IL-6, and murine IL-3 and SCF. (C) New BFU-E formation after replating of primary BFU-Es (n = 3 in each). (D) CFU-E formation from BM cells in the absence (n = 4 in each) and presence (n = 3 in each) of EPO.*P < .05, **P < .01, ***P < .001.
