Hmga2 transgenic mice. (A) Diagram of 3′UTR-truncated Hmga2 (ΔHmga2) lacking 6 of 7 complementary sites of let-7-family microRNAs. BP shows the break point as it has been described in patients with PNH.⁶  The phosphoglycerate kinase-1 promoter (PGK-PROM) and polyadenylate tail (PGK-PA) served to express HMGA2 protein, integrated into chromosome 9E3.3, 5.4 kb from end of Clstn2 and 315.2 kb from end of Nmnat3. (B) Body weight of 3-month-old ΔHmga2 mice (male, n = 5; female, n = 4) compared with that of WT mice (male and female, n = 5 each). (C) Increased expression of Hmga2 mRNA in ΔHmga2 mice (n = 4) compared with WT mice (n = 3) as determined by QRT-PCR analysis. (D-E) Western blots of HMGA2 protein expression. Results of spleen, thymus, and liver derived from a single gel, and a vertical line between WT and ΔHmga2 has been inserted to indicate a repositioned gel lane (D). Results of BM derived from a single gel (E). Representative data of 3 independent experiments are shown. *P < .05.