Analyses of TCR and IGH rearrangement in BM and CB iPSC lines. (A) PCR analyses of TCRB rearrangements. (B) PCR analyses of TCRG rearrangement. (C) PCR analyses of IGH rearrangements. FR indicates framework. (D) Specimen controls. M indicates the 50-bp DNA ladder; H2O, no template control; H1, genomic DNA from hESC H1 (negative control). Eleven iPSC lines were examined as indicated. IVS-0000 is a polyclonal control DNA; IVS-0009, IVS-0004, IVS-0021, IVS-0030, and IVS-0029 are clonal control DNAs.