Reprogramming of CB mononuclear cells with nonintegrating constructs. (A) All 22 CB iPSC lines express hESC-specific surface markers as indicated, and express OCT4, NANOG, and SOX2. iPSC lines checked are: CB iPSC1 to CB iPSC6, CB iPSCT1 to CB iPSCT10, CB iPSCT12 to CB iPSCT16, and CB iPSCND. (B) Thiazovivin (T+) promotes reprogramming of CB mononuclear cells. The numbers of iPSC lines generated from 1.7 × 106 transfected CB mononuclear cells are shown. (C) Normal karyogram of the CB iPSC6 line. (D) H&E staining of representative terotoma from CB iPSCs with derivatives of 3 germ layers. (E-G) CB iPSCs cells are free of transgene and episomal DNA. Episomal DNA (E) and genomic DNA (F) were prepared from CB iPSC lines of CB iPSC1, CB iPSC6, and CB iPSCT3, -4, -7, -8, and -9. RT-PCR analysis of expression of transgenes and endogenous pluripotency genes. T-transgene–specific primers were used as indicated.
