Detection of FG bound to nHZ. (A) Coomassie stain of 20 μg of nHZ proteins separated by a 7%-17% gradient SDS-PAGE (corresponding to 50nmol heme/lane in terms of heme content). nHZ was isolated from P falciparum in vitro cultures in RPMI-1640 growth medium supplemented with 10% plasma. (B) Western blot of 10 μg of nHZ proteins separated by 10% SDS-PAGE transferred to nitrocellulose and probed with a mouse monoclonal anti-FG γ-chain and a peroxidase-linked secondary Ab. FG chains were detected by ECL. Solubilized FG (15 μg) was run in parallel as standard. One representative protein separation in SDS-PAGE and Western blot of 15 and 6, respectively, are shown.