Response of Crf1 peptide-specific T-cell clones to stimulation with native A fumigatus antigen. (A) T-cell clones from different donors specific for p1, p22, p27, p41, and p59 that responded to stimulation with Crf1 mRNA-transfected DCs were stimulated with A fumigatus cell extract or (B) inactivated germinating A fumigatus conidia (n = 3). Shown is 1 clone per antigen and donor. (C) Crf1/p41-specific T-cell clones respond to stimulation with DCs incubated with germinating A fumigatus conidia (6 hours) or hyphae (12 hours) but not or only weakly to resting conidia (0 hours; n = 3).