Gene expression analysis of T cells obtained from enlarged thymi of ERG mice and Southern blot analyses of control and ERG mice. (A) Gene Set Enrichment Analysis showing enrichment of genes regulated by Notch1. (B) Quantitative PCR analysis of transcripts of Notch1-regulated genes, Notch1 itself, Myc, Hes1, and Deltex1 (Dtx1). Results are presented as a fold change compared with control thymocytes after normalization on the basis of transcript levels of β-actin. Results (mean and SD) obtained from 3 mice are shown. (C) Nonerythroid/non-T cells (NET) and erythroid cells (E) were fractionated from BM and spleen of the indicated mice. T cells were obtained from thymi (T). Two GFP-only control and 4 ERG mice were used. Genomic DNA extracted from respective cells was analyzed for virus integration by a GFP probe. The “a” to “f” indicate mice shown in Figure 1G. Mutation status of Notch1 is also shown. WT indicates wild type; MT, mutated.